Molecular Genetics of Prokaryotes Problem SetProblem 5: DNA transfer by an F factorAn E. coli strain is F lac Z met bio. Cells from this strain are mixed with an E. coli strain that is lac Z met bio and carrying an F' episome with the Plac O lac Z DNA sequence on the episome, and cultured for several hours. Then cells were removed, washed, and transferred to minimal media containing lactose as the only sugar source. A few cells were able to grow on minimal media with lactose, and formed colonies. How did these few cells become lac Z met bio?
University of Arizona Updated: July 15, 1999 Contact the Development Team
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