Molecular Genetics of Prokaryotes Problem Set

Problem 7: Regulation of lac Z gene on an F' episome

How would you describe the regulation of lactose metabolism in the cells described in problem 5 that were able to grow on minimal media with lactose as a nutrient?

A. normal regulation of lactose metabolism
Plac O lac Z DNA sequence on the episome is regulated by the repressor and CRP protein encoded on the bacterial chromosome.
B. constitutive expression of lac Z
C. inability to synthesize the lac Z gene product, ß-galactosidase
D. lac Z gene is inducible, but unable to be repressed by high glucose
E. no synthesis of the lac I gene product, the lac repressor


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