Molecular Genetics of Prokaryotes Problem Set Problem 5: DNA transfer by an F factor An E. coli strain is F lac Z met bio. Cells from this strain are mixed with an E. coli strain that is lac Z met bio and carrying an F' episome with the Plac O lac Z DNA sequence on the episome, and cultured for several hours. Then cells were removed, washed, and transferred to minimal media containing lactose as the only sugar source. A few cells were able to grow on minimal media with lactose, and formed colonies. How did these few cells become lac Z met bio? A. transformation B. transduction C. sexduction, a special type of conjugation The process by which autonomous pieces of DNA are carried into an F bacterium by an F factor DNA is called sexduction. D. conjugation E. transposition The Biology Project University of Arizona Updated: July 15, 1999 Contact the Development Team http://www.biology.arizona.edu All contents copyright © 1998-99. All rights reserved.