ELISA Activity

Question 3

What would happen if the anti-human Ig-conjugate were not washed free of the well before the substrate was added?

The ELISA would not develop when the substrate was added.

Incorrect.
The substrate would over-develop because an excess of enzyme coupled to the anti-human Ig was present.

The Biology Project
University of Arizona
Monday, March 31, 1997
denicew@u.arizona.edu