ELISA Activity
Problem 3
What would happen if the anti-human Ig-conjugate were not washed free of the well before the substrate was added?
The ELISA would not develop when the substrate was added. The ELISA would develop normally. All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate. Both A and B.
The University of Arizona
Wednesday, January 22, 1998
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