ELISA Activity

Question 3

What would happen if the anti-human Ig-conjugate were not washed free of the well before the substrate was added?
The ELISA would develop normally.
Incorrect.
The substrate would over-develop because an excess of enzyme coupled to the anti-human Ig was present.


The Biology Project
University of Arizona
Monday, March 31, 1997
denicew@u.arizona.edu

http://www.biology.arizona.edu
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